Inhibiting Apoptosis of CTLL-2 Cells to Enhance their GVL Effects via Anti-Fas Ribozyme
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چکیده
منابع مشابه
Inhibiting apoptosis of CTLL-2 cells to enhance their GVL effects via anti-Fas ribozyme.
To investigate the inhibition role of anti-Fas hammerhead ribozyme on fas expression and Fas-mediated apoptosis of CTL cell line CTLL-2 cells, the cDNA of an anti-Fas hammerhead ribozyme was synthesized, its expression plasmid was constructed and transfected into CTLL-2 cells by electroporation. fas expression of CTLL-2 cells was detected by RT-PCR and Western blot. CTLL-2 cell viability was me...
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To detect a new and more effective way against apoptosis mouse lymphomatic cell line Yac-1 in which fas gene was expressed highly was used as a model for studying the effects of anti-Fas ribozyme on Fas-mediated apoptosis. A hammerhead ribozyme gene targeting the fas mRNA was synthesized and its in vitro transcription vector was constructed, which was transfected into Yac-1 cells using electrop...
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ژورنال
عنوان ژورنال: Acta Biochimica et Biophysica Sinica
سال: 2004
ISSN: 1745-7270
DOI: 10.1093/abbs/36.8.559